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Inhibition of aldose reductase and anti-cataract action of trans-anethole isolated from Foeniculum vulgare Mill. fruits

Dongare, Vandana, Kulkarni, Chaitanya, Kondawar, Manish, Magdum, Chandrakant, Haldavnekar, Vivek, Arvindekar, Akalpita
Food chemistry 2012 v.132 no.1 pp. 385-390
Foeniculum vulgare, Lens, alcohol oxidoreductases, animal disease models, blood glucose, catalase, cataract, distillates, enzyme inhibition, eye lens, fractionation, fruits, glucose, glutathione, glycemic effect, hemoglobin, high performance liquid chromatography, lipids, medicinal properties, rats, silica gel, superoxide dismutase
Foeniculum vulgare fruits are routinely consumed for their carminative and mouth freshening effect. The plant was evaluated for aldose reductase inhibition and anti-diabetic action. Bioguided fractionation using silica gel column chromatography, HPLC, and GC–MS analysis revealed trans-anethole as the bioactive constituent possessing potent aldose reductase inhibitory action, with an IC₅₀ value of 3.8μg/ml. Prolonged treatment with the pet ether fraction of the F. vulgare distillate demonstrated improvement in blood glucose, lipid profile, glycated haemoglobin and other parameters in streptozotocin-induced diabetic rats. Trans-anethole could effectively show anti-cataract activity through the increase in soluble lens protein, reduced glutathione, catalase and SOD activity on in vitro incubation of the eye lens with 55mM glucose. Trans-anethole demonstrated noncompetitive to mixed type of inhibition of lens aldose reductase using Lineweaver Burk plot.