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Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium

Behrendt, Undine, Augustin, Jürgen, Spröer, Cathrin, Gelbrecht, Jörg, Schumann, Peter, Ulrich, Andreas
Antonie van Leeuwenhoek 2015 v.108 no.6 pp. 1457-1468
Proteus penneri, Proteus vulgaris, carbon nitrogen ratio, community structure, grasslands, interspecific variation, matrix-assisted laser desorption-ionization mass spectrometry, new species, nitrate nitrogen, nitrate reduction, nitrogen, nitrous oxide, nucleic acid hybridization, nucleotide sequences, peat, phylogeny, ribosomal RNA, soil, soil bacteria
In the context of studying the influence of N-fertilization on N₂ and N₂O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N₂O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA–DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687ᵀ =DSM 29910ᵀ =LMG 28659ᵀ) is proposed.