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Impact of plant extracts tested in attention-deficit/hyperactivity disorder treatment on cell survival and energy metabolism in human neuroblastoma SH-SY5Y cells

Schmidt, Andreas Johannes, Krieg, Jürgen-Christian, Hemmeter, Ulrich Michael, Kircher, Tilo, Schulz, Eberhard, Clement, Hans-Willi, Heiser, Philip
Phytotherapy research 2010 v.24 no.10 pp. 1549-1553
Hypericum perforatum, Pinus, adenosine triphosphate, bark, clinical trials, cytotoxicity, drugs, energy metabolism, humans, neuroprotective effect, plant extracts
Plant extracts such as Hypericum perforatum and Pycnogenol® have been tested as alternatives to the classical ADHD drugs. It has been possible to describe neuroprotective effects of such plant extracts. A reduction of ADHD symptoms could be shown in clinical studies after the application of Pycnogenol®, which is a pine bark extract. The impacts of the standardized herbal extracts Hypericum perforatum, Pycnogenol® and Enzogenol® up to a concentration of 5000 ng/mL on cell survival and energy metabolism in human SH-SY5Y neuroblastoma cells has been investigated in the present examination. Hypericum perforatum significantly decreased the survival of cells after treatment with a concentration of 5000 ng/mL, whereas lower concentrations exerted no significant effects. Pycnogenol® induced a significant increase of cell survival after incubation with a concentration of 32.25 ng/mL and a concentration of 250 ng/mL. Other applied concentrations of Pycnogenol® failed to exert significant effects. Treatment with Enzogenol® did not lead to significant changes in cell survival.Concerning energy metabolism, the treatment of cells with a concentration of 5000 ng/mL Hypericum perforatum led to a significant increase of ATP levels, whereas treatment with a concentration of 500 ng/mL had no significant effect. Incubation of cells with Pycnogenol® and Enzogenol® exerted no significant effects.None of the tested substances caused any cytotoxic effect when used in therapeutically relevant concentrations.