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Protective Effect of Porcine Cerebral Hydrolysate Peptides on Learning and Memory Deficits and Oxidative Stress in Lead-Exposed Mice

Zou, Ye, Feng, Weiwei, Wang, Wei, Chen, Yao, Zhou, Zhaoxiang, Li, Qian, Zhao, Ting, Mao, Guanghua, Wu, Xiangyang, Yang, Liuqing
Biological trace element research 2015 v.168 no.2 pp. 429-440
memory, matrix-assisted laser desorption-ionization mass spectrometry, locomotion, lead, reactive oxygen species, lead acetate, blood, oxidative stress, protective effect, glutathione, malondialdehyde, brain, iron, nitric oxide synthase, ultrasonics, mice, subtilisin, hydrolysates, swine, zinc, absorption
In this study, lead acetate solution and porcine cerebral hydrolysate peptides (PCHPs) were administered to developing mice. Porcine cerebral protein pretreated by ultrasound was hydrolyzed with alcalase, and 11 peptide fragments were obtained by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of PCHPs. Our data showed that PCHPs significantly decreased Pb²⁺-induced spontaneous locomotor activity, latencies to reach the platform, and the time in target quadrant. It also decreased the accumulation of lead in the blood and brain of Pb²⁺-exposed developing mice. Co-administration of PCHPs and dimercaptosuccinic acid (DMSA) did not only reduce the accumulation of lead in blood but also increased the absorption of zinc and iron in Pb²⁺-exposed mice. Administration of PCHPs individually significantly enhanced hematopoietic parameters compared with the Pb²⁺-exposed group. PCHPs significantly reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) but increased glutathione (GSH) content and anti-oxidant enzymes and nitric oxide synthase (NOS) activities in Pb²⁺-exposed brain. Our findings suggest that PCHPs have the ability to protect against Pb²⁺-exposed learning and memory deficits and oxidative damage.