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Effect of Caseinophosphopeptides from Î±s- and Î²-Casein on Iron Bioavailability in HuH7 Cells
- Garcia-Nebot, Maria Jose, Alegria, Amparo, Barbera, Reyes, Gaboriau, Francois, Bouhallab, Said
- Journal of agricultural and food chemistry 2015 v.63 no.30 pp. 6757-6763
- alphaS1-casein, alphaS2-casein, beta-casein, bioavailability, ferritin, hepatocytes, hepatoma, humans, transferrin
- Two pools of caseinophosphopeptides (CPPs) obtained from Î±s- and Î²-casein fractions (Î±-CPPs and Î²-CPPs) were characterized. A total of 16 CPPs were identified in the Î±-CPPs pool, 9 of them derived from Î±s1-casein and 7 from Î±s2-casein. A total of 18 CPPs were identified in the Î²-CPPs pool. Four of the identified CPPs contained the characteristic phosphoseryl-glutamic acid cluster SpSpSpEE. Calcein assay was used to compare the iron-binding capacity of the Î±- and Î²-CPPs pools. At the concentration of 12.5 Î¼M CPPs used in the iron bioavailability assays, Î²-CPPs pools show greater iron-binding capacity than Î±-CPPs pools. HuH7 human hepatoma cells show many differentiated functions of liver cells in vivo and can be used to evaluate iron bioavailability (ferritin content and soluble transferrin receptor) from FeâÎ±-CPPs and FeâÎ²-CPPs complexes. The Î±-CPPs and Î²-CPPs pools did not improve ferritin content or soluble transferrin receptor in HuH7 cells.