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Identification of the centromeric repeat in the threespine stickleback fish (Gasterosteus aculeatus)
- Cech, Jennifer N., Peichel, Catherine L.
- Chromosome research 2015 v.23 no.4 pp. 767-779
- Gasterosteus aculeatus, Y chromosome, centromeres, fish, fluorescence in situ hybridization, fluorescent antibody technique, genome, high-throughput nucleotide sequencing, interphase, metaphase, precipitin tests
- Centromere sequences exist as gaps in many genome assemblies due to their repetitive nature. Here we take an unbiased approach utilizing centromere protein A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to identify the centromeric repeat sequence in the threespine stickleback fish (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric using both fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. Together, our work provides the first validated sequence information for the threespine stickleback centromere.