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Impact of immature fruit size and genotype on direct Caullogenesis in Monoembryonic-seed mango
- Abul-Soad, A. A., Markhand, G. S., Solangi, N. D.
- Acta horticulturae 2015 no.1083 pp. 85-94
- 2,4-D, activated carbon, antioxidants, callus, cultivars, culture media, explants, genotype, mangoes, micropropagation, nucellus, plantlets, rootstocks, seeds, shoots
- The improved in vitro protocol of monoembryonic mango would facilitate the commercial micropropagation of cultivars and rootstocks. The nucellus tissue of mono-embryonic seeds of world-known mango cultivars âSaroliâ, âLangraâ and âChaunsaâ were used to induce the direct shoots. The simple procedure used for surface sterilization of the immature fruit resulted in 95% free-contaminants explants. The initial fruit size was found to be crucial for getting the DS within a short period of time (2 months). Full half of seeds from immature fruit without zygotic embryo were used as an initial explant. Browning of initial explants was controlled by anti-oxidants solution, activated charcoal containing-media and incubation in dark till maturation. The developed medium formula to induce cluster of shoots (0.5 cm long) was micro-salts of MS and macro of B5 supplemented with 4.52 Î¼M 2,4-D and 9.83 Î¼M 2iP within 3 months under dark. The appropriate fruit size used to induce the DS (caullogenesis) was 5-6 cm long. The smaller fruit size 1 cm long could not develop and died while the fruit size 2-4 cm long developed clusters of embryogenic callus under dark and 27Â±2Â°C. The induced clusters of shoots were cultured on the basal medium supplemented with 2.3 Î¼M Kin and 0.49 Î¼M 2iP in order to grow into small healthy plantlets under light and 27Â±2Â°C. Caullogenesis was detected only in âLangraâ and âChaunsaâ whereas âSaroliâ explants failed throughout the two seasonsâ experiment. The step-wise protocol and morphology of the induced DS were described in the current study.