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Characterization of one trinucleotide and six dinucleotide microsatellite markers in bicolor damselfish, Stegastes partitus, a common coral reef fish

Author:
Thiessen, Roger J., Heath, Daniel D.
Source:
Conservation genetics 2007 v.8 no.4 pp. 983-985
ISSN:
1566-0621
Subject:
Pomacentridae, alleles, corals, fish, heterozygosity, loci, microsatellite repeats
Abstract:
Fourteen primer pairs were designed from 47 sequences containing microsatellites isolated from agenomic library enriched for (GACA)₄ in Stegastes partitus. Seven of these were shown to be polymorphicand provided clean amplification products. These seven loci were further characterized using 38 individuals, yielding a range in number of alleles from 5 to 31 and observed and expected heterozygosity values rangingfrom 0.45-0.89 and 0.66-0.96 respectively. Six of seven loci conformed to Hardy-Weinberg equilibrium.Successful amplification was also achieved in other Pomacentridae.
Agid:
468545