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Effects of photomixotrophic conditions and type of culture vessel closure on Anacardium othonianum Rizz. grown in vitro

Assis, K. C. de, Silva, F. G., Pereira, F. D., Vasconcelos-Filho, S. C., Menezes, C. C. de
Acta horticulturae 2015 no.1083 pp. 553-564
Anacardium, activated carbon, agar, cerrado, cotton, farmers, fruit trees, indole butyric acid, leaves, plantlets, shoots, sucrose, woody plants
Anacardium othonianum Rizz. is a species of fruit tree from the Brazilian cerrado; the economical exploitation of which is a viable alternative for local farmers. The present study aimed to evaluate the influence of sucrose and the type of culture tube closure on the in vitro leaf anatomy of A. othonianum. Shoots were transferred to glass tubes containing woody plant medium supplemented with 5.9 µM indole-3-butyric acid, 4 g L-1 agar and 2 g L-1 activated charcoal, in the absence or presence of sucrose (3%). The tubes were closed with plastic caps, plastic caps wrapped with PVC film, or with cotton plugs. The anatomical variables were larger under heterotrophic conditions, but tended to decrease when a photomixotrophic stimulus was applied. However, by maintaining the plantlets under photomixotrophic conditions in tubes closed with cotton wool plugs, the values of the anatomical variables diminished, thus improving the chances of success in the subsequent stages of propagation. The results obtained revealed that A. othonianum exhibited a considerably adaptive plasticity. By diminishing the values of the anatomical variables, cotton wool closures provided not only an adequate environment for the growth of plantlets in vitro under photomixotrophic conditions.