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Environmental Monitoring of Mycobacterium bovis in Badger Feces and Badger Sett Soil by Real-Time PCR, as Confirmed by Immunofluorescence, Immunocapture, and Cultivation
- Sweeney, F.P., Courtenay, O., Hibberd, V., Hewinson, R.G., Reilly, L.A., Gaze, W.H., Wellington, E.M.H.
- Applied and environmental microbiology 2007 v.73 no.22 pp. 7471-7473
- Mycobacterium bovis, badgers, cattle, environmental monitoring, feces, fluorescent antibody technique, polymerase chain reaction, soil, taxonomy, viability, wildlife
- Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.