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Cryopreservation of brown algae gametophytes of Undaria pinnatifida by encapsulation–vitrification

Author:
Wang, Bing, Zhang, Endong, Gu, Yue, Ning, Shuxiang, Wang, Qihua, Zhou, Jiti
Source:
Aquaculture 2011 v.317 no.1-4 pp. 89-93
ISSN:
0044-8486
Subject:
Undaria pinnatifida, cryopreservation, gametophytes, glycerol, sporophytes, sucrose, viability, washing
Abstract:
The cryopreservation of gametophytes of Undaria pinnatifida by encapsulation–vitrification was studied. The effect of the composition of the vitrification solution (VS), the composition and concentration of the loading solution (LS), the duration of loading and dehydration treatments and the washing method on viability of algae were investigated. The highest viability index for female and male gametophytes was 31.2% and 26.0%, respectively, which were obtained by loading alginate beads with a mixture of 2M glycerol and 0.6M sucrose for 120 and 90min at 25°C, dehydrated with PVS2 for 50 and 40min at 0°C and washed with a 1.2M sucrose solution. Intact sporophytes developed from gametophytes cryopreserved have been observed.
Agid:
470875