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Effects of dietary pyridoxine on haemato‐immunological responses of Labeo rohita fingerlings reared at higher water temperature

Akhtar, M. S., Kumar Pal, A., Sahu, N. P., Ciji, A., Kumar, N.
Journal of animal physiology and animal nutrition 2012 v.96 no.4 pp. 581-590
Labeo rohita, ambient temperature, crude protein, diet, erythrocytes, fingerlings, hemoglobin, leukocytes, pyridoxine, serum albumin, water temperature, weight gain
A sixty‐day feeding trial was carried out to elucidate the effect of dietary pyridoxine (PN) on hemato‐immunological parameters in Labeo rohita fingerlings exposed to an elevated temperature (ET) of 33 °C. Two hundred and seventy fingerlings (6.71 ± 0.32 g) were randomly distributed into six treatments in triplicates (15 fish/tank). Five iso‐nitrogenous (356.3 ± 2.7 g/kg crude protein) purified diets were prepared with graded levels (0, 10, 50, 100 and 200 mg/kg diet) of PN. Six treatment groups were P10T26 (10 mg PN + ambient temperature (26 °C), P0T33 (0 mg PN + ET), P10T33 (10 mg PN + ET), P50T33 (50 mg PN + ET), P100T33 (100 mg PN + ET) and P200T33 (200 mg PN + ET). The responses of L. rohita fingerlings to dietary PN were assessed in terms of growth and by analysing some hemato‐immunological parameters. When PN supplementation increased from 10 to 200 mg/kg diet, a significant improvement in weight gain percentage was observed. Erythrocyte (RBC) count, leucocyte (WBC) count, haemoglobin content and respiratory burst activity were significantly (p < 0.05) higher in 100 mg PN/kg diet–fed group than their non‐PN‐fed counterpart. Also, serum albumin, globulin and lysozyme activity were found significantly (p < 0.05) higher in PN‐fed groups. The overall results indicated that dietary PN supplementation at 100 mg/kg diet may reverse the negative effects caused by ET and may protect the haemato‐immunological status of L. rohita fingerlings reared at higher water temperature.