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New primers for single-copy nuclear-encoded chloroplast-expressed glutamine synthetase (ncpGS) in Oxalidaceae

Author:
Oberlander, Kenneth C., Dreyer, Léanne L., Roets, Francois
Source:
American journal of botany 2010 v.97 no.12 pp. e146
ISSN:
0002-9122
Subject:
Oxalis, glutamate-ammonia ligase, sequence homology
Abstract:
Premise of the study: New primers were developed for the nuclear marker glutamine synthetase (ncpGS) in Oxalidaceae. METHODS: and Results: New forward and reverse primers were designed and tested across a wide range of Oxalidaceae. Selected taxa were sequenced to confirm homology. Potential for phylogenetic study was assessed by comparing sequenced taxa with commonly used nuclear and plastid markers. CONCLUSIONS: Four out of five Oxalidaceae genera and all tested Oxalis spp. amplified successfully. Sequencing confirmed homology of the amplicon. Parsimony analysis of ncpGS showed that it is a promising candidate for future phylogenetic work in Oxalidaceae.
Agid:
475105