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High-throughput microsatellite marker development in Amur catfish (Silurus asotus) using next-generation sequencing
- Xu, Dandan, Zhang, Yaoguang, Peng, Zuogang
- Conservation genetics resources 2013 v.5 no.2 pp. 487-490
- Silurus, alleles, catfish, loci, microsatellite repeats, East Asia, Yangtze River
- The Amur catfish, Silurus asotus, is an economically important fish species in East Asia, but few genetic studies have been conducted on this species, especially those based on nuclear markers. Here, we isolated and characterized 47 novel polymorphic microsatellite loci in the genome of S. asotus using 454 sequencing. We screened 70 primers and 48 of them generated amplification products. Forty-seven of the amplification products were polymorphic in a population of 40 collected from the upper Yangtze River. The number of alleles varied from 3 to 15, and the observed and expected heterozygosities varied from 0.300 to 0.800 and 0.305 to 0.866, respectively. The average polymorphic information content (PIC) of all loci was 0.682, indicating high levels of polymorphism. In addition, cross-species amplification in a congener species, Silurus meridionalis showed a high level of transferability (79.2 %), which confirmed that the microsatellite markers developed here could be used effectively for other related catfish species.