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Derivatisation for separation and detection in capillary electrophoresis (2012–2015)
- Wuethrich, Alain, Quirino, Joselito P.
- Electrophoresis 2016 v.37 no.1 pp. 45-55
- capillary electrophoresis, chemists, derivatization, wavelengths
- Derivatisation is a well‐established and mature form of sample preparation for CE. The modification of the analyte can cause superior analysis characteristics such as better sensitivity and selectivity, however, derivatisation of the analyte introduces an additional step into the analytical workflow. This review covers articles from January 2012 to January 2015 on derivatisation in CE. The main sections are on the derivatisation modes (i.e. pre‐capillary, in‐line, in‐capillary and post‐capillary), separation and detection modes (i.e. LIF and others). LIF is discussed in more detail since this detection mode was most prevalent. A table of the common labelling agents and wavelengths for excitation and emission and the common derivatisation reactions are included. In addition, a comprehensive table which summarises all research articles is provided. This review is suitable for analytical chemists as a guide for ‘how to get started’ with derivatisation for separation and detection in CE.