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The matrix interference to the immunoassay of food samples: The effect of some proteins in aquatic products

Author:
Wang, Xiudan, Lin, Hong, Cao, Limin, Zhang, Xue, Sui, Jianxin
Source:
Food and agricultural immunology 2016 v.27 no.2 pp. 230-241
ISSN:
1465-3443
Subject:
Pleuronectiformes, Western blotting, antibodies, fish, immunoassays, proteins, shrimp
Abstract:
The elimination of matrix interference is now a task for further development and application of immunoassays. Here the effect of proteins in aquatic samples on immunoassay was investigated by western blotting and competitive indirect enzyme-labeled immunosorbent assay (ci-ELISA). Some proteins in flatfishes interacted with different antibodies, resulting in significant loss of accuracy of the immunoassays. Moreover, these matrix proteins prefer to interact with enzyme-labeled antibodies than label-free ones. Such a matrix protein–immunoglobulin interaction was validated with different fish and shrimp samples and similar results were observed, which demonstrated a universal significance of the protein-induced matrix interference to immunoassays. Besides, the strategies to prevent such interference were preliminarily discussed.
Agid:
4780980