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A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells

Author:
Kang, Yun Gyeong, Shin, Ji Won, Park, So Hee, Kim, Young Mi, Gu, Seo Rin, Wu, Yanru, Ban, Hun Yeong, Shin, Jung-Woog
Source:
Biotechnology letters 2016 v.38 no.1 pp. 175-181
ISSN:
0141-5492
Subject:
biomimetics, blood, extracellular matrix, phenotype, stem cells, umbilical cord
Abstract:
OBJECTIVE: To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds. RESULTS: The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45⁺CD34⁺ (35 %) and CD34⁺CD38⁻ (32 %) ratios. CONCLUSION: Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.
Agid:
4781337