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Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro

Author:
Miszkiel, Grażyna, Kotwica, J.
Source:
Acta veterinaria Hungarica 2001 v.49 no.1 pp. 39-51
ISSN:
1588-2705
Subject:
agonists, estrous cycle, heifers, luteal cells, mechanism of action, norepinephrine, oxytocin, pregnenolone, progesterone, prostaglandins, secretion, steroidogenesis
Abstract:
The present studies were conducted: (1) to determine which β-adrenoceptor subtypes are involved in progesterone and oxytocin (OT) secretion, (2) to examine whether noradrenaline (NA) acts directly on the cytochrome P-450scc and 3β-hydroxysteroid dehydrogenase (3β-HSD), and (3) to study the effect of prostaglandin F ₂α, (PGF ₂α) on NA-stimulated steroidogenesis in luteal cells. The effect of NA on progesterone secretion from luteal slices of heifers on days 8–12 of the oestrous cycle was blocked by both atenolol (β ₁-antagonist) and ICI 118.551 hydrochloride (β2-antagonist). OT secretion was blocked only after treatment with ICI 118.551 hydrochloride (P < 0.05). Dobutamine (10 ⁻⁴−10 ⁻⁶), a selective β ₁ agonist and salbutamol (10 ⁻⁴−10 ⁻⁶), a selective β ₂ agonist, both increased progesterone production (P < 0.01) with an efficiency comparable to that produced by NA (P < 0.01). The increase of OT content in luteal slices was observed only after treatment with salbutamol at the dose of 10 ⁻⁵M (P < 0.01). Dobutamine had no effect on OT production at any dose. A stimulatory effect of NA on cytochrome P-450scc activity (P < 0.05) was demonstrated using 25-hydroxycholesterol as substrate. 3β-HSD activity also increased following NA (P < 0.01) or pregnenolone (P < 0.05) and in tissue treated with pregnenolone together with NA (P < 0.01). PGF decreased progesterone synthesis (P < 0.05) and 3β-HSD activity (P < 0.01) in tissue treated with NA. We conclude that NA stimulates progesterone secretion by luteal β ₁- and β ₂-adrenoceptors, while OT secretion is probably mediated only via the β ₂-receptor. NA also increases cytochrome P-450scc and 3β-HSD activity. PGF inhibits the luteotropic effect of NA on the luteal tissue.
Agid:
4784370