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Porcine theca cells produce immunoreactive β-endorphin and change steroidogenesis in response to opioid agonist

Kamiński, T., Okrasa, S., Bogacka, Iwona, Siawrys, Gabriela, Przała, Jadwiga
Acta veterinaria Hungarica 2001 v.49 no.3 pp. 319-329
agonists, androstenedione, antagonists, culture media, estradiol, granulosa cells, in vitro studies, luteinizing hormone, naloxone, narcotics, opioid peptides, progesterone, secretion, steroidogenesis, swine, testosterone
In earlier in vitro experiments opioids affected steroidogenesis in porcine luteal and granulosa cells. The present studies were undertaken to examine the effects of FK 33-824 (opioid agonist) alone or in combination with LH, PRL or naloxone (NAL, opioid antagonist) on steroidogenesis in cultured porcine theca cells. Moreover, we have tested β-endorphin-like immunoreactivity (β-END-LI) concentrations in culture media under control conditions and following treatments of theca cells with LH, PRL, progesterone (P ₄), oestradiol (E ₂) or testosterone (T). FK 33-824 and NAL significantly increased P ₄ release by theca cells and inhibited stimulatory effect of LH on this steroid output. PRL-induced P ₄ secretion from the cells was blunted only by FK 33-824. Secretion of androstenedione (A ₄) and T was essentially elevated in the presence of FK 33-824 and this potentiation of both androgen release was completely abolished by PRL. NAL blocked stimulatory effect of the opioid agonist only in case of T. Secretion of oestradiol and oestrone was completely free from the influence of both the opioid agonist and antagonist. Pig theca cells were able to produce β-END-LI but none of tested hormones (LH, PRL, P ₄, E ₂ and T alone or in combination) significantly affected this production. In conclusion, these data indicate that porcine theca cells may produce β-END-LI and change their steroidogenesis in response to opioid peptides.