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Comparative mapping and QTL analysis of early spring adaptation traits in barley (Hordeum vulgare L.)

Author:
Karsai, I., Mészáros, K., Szűcs, P., et al.,
Source:
Acta agronomica Academiae Scientiarum Hungaricae 2002 v.50 no.3 pp. 283-294
ISSN:
0238-0161
Subject:
Hordeum vulgare, barley, chromosome mapping, genes, genetic background, germplasm, heading, marker-assisted selection, molecular cloning, overwintering, phenotype, quantitative trait loci, spring
Abstract:
The possibilities latent in molecular marker-based QTL analyses are presented through the example of studying winter survival and heading date in barley (<i>Hordeum vulgare</i> L.). The whole range of QTL experiments consists of several important steps, through which answers are found to the following questions: (1) How many QTLs are involved and where do they map, (2) How does the environment influence the effect of a QTL region (environment × QTL interactions), (3) When and where are the genes determining the given trait expressed (QTL dynamics), (4) What interactions occur between these QTLs and pathways leading to specific phenotypes, and (5) How consistent is the effect of a QTL region in different genetic backgrounds and in a wider range of germplasms (comparative mapping and association studies)? This knowledge then makes it possible to continue these experiments in the direction of marker-assisted selection and/or gene isolation through marker saturation of the relevant chromosomal regions and map-based cloning. The latter can give an insight into the exact mechanism through which the gene determines the phenotype.
Agid:
4784621