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Development of nuclear microsatellite markers for the fonio, Digitaria exilis (Poaceae), an understudied West African cereal

Barnaud, Adeline, Vignes, Hélène, Risterucci, Ange-Marie, Noyer, Jean-Louis, Pham, Jean-Louis, Blay, Carole, Buiron, Marylène, Vigouroux, Yves, Billot, Claire
American journal of botany 2012 v.99 no.3 pp. e105
Digitaria exilis, alleles, genetic variation, grains, heterozygosity, loci, microsatellite repeats, population genetics, population structure, Western Africa
• Premise of the study: We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa. • METHODS: and Results: We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, five tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93. • CONCLUSIONS: The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio.