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Composition and biological activities of slaughterhouse blood from red deer, sheep, pig and cattle

Author:
Bah, Clara SF, Bekhit, Alaa El‐Din A, Carne, Alan, McConnell, Michelle A
Source:
Journal of the science of food and agriculture 2016 v.96 no.1 pp. 79-89
ISSN:
0022-5142
Subject:
2,2-diphenyl-1-picrylhydrazyl, Cervus elaphus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, anti-infective properties, antioxidant activity, blood meal, blood proteins, blood serum, cattle, deer, erythrocytes, humans, iron, livestock and meat industry, neutrophils, peptidyl-dipeptidase A, reactive oxygen species, sheep, slaughterhouses, swine
Abstract:
BACKGROUND: Animal blood is a large‐volume by‐product of the meat industry. Besides blood meal fertiliser, blood is marketed for human consumption as a supplement. Minimal comparative work on slaughterhouse animal blood fractions has been carried out. In this study, slaughterhouse deer, sheep, pig and cattle blood parameters were compared. Some blood constituents were determined. Fractionated blood was assessed for antioxidant activity (2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging, oxygen radical scavenging capacity and ferric reducing antioxidant power). Angiotensin converting enzyme (ACE) inhibitory activity and antimicrobial activity were also assessed. RESULTS: Serum iron ranged from 35.3 ± 0.6 µmol L⁻¹ in cattle to 16.3 ± 3.1 µmol L⁻¹ in deer. Cattle had the highest total plasma proteins (81.7 ± 1.5 g L⁻¹). While the plasma fractions contained considerable antioxidant activity, the red blood cell fractions of all four animal species contained higher antioxidant activity (P < 0.05). Negligible levels of ACE inhibitory activity were found for all animal blood fractions. Antimicrobial activity was detected towards Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa with sheep white blood cells from which a crude neutrophil extract was obtained which demonstrated concentration‐dependent inhibitory effects on the growth rates of these bacterial strains. CONCLUSION: Fractionated animal blood obtained from local slaughterhouses contains native proteins that possess antioxidant activity and antimicrobial activity. © 2015 Society of Chemical Industry
Agid:
4789510