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Nondenaturing polyacrylamide gel electrophoresis to study the dissociation of the p53·MDM2/X complex by potentially anticancer compounds

Author:
Sgammato, Roberta, Desiderio, Doriana, Lamberti, Anna, Raimo, Gennaro, Novellino, Ettore, Carotenuto, Alfonso, Masullo, Mariorosario
Source:
Electrophoresis 2015 v.36 no.24 pp. 3101-3104
ISSN:
0173-0835
Subject:
antineoplastic agents, dissociation, gels, mice, polyacrylamide, polyacrylamide gel electrophoresis
Abstract:
A new analytical method to study the dissociation of the complexes between the oncosuppressor p53 and its negative modulators murine double‐minute protein 2 (MDM2) or MDMX, is proposed. This technique is reliable to determine the dissociative power exerted by small molecules on the complex taking advantage of the appearance of migrating MDM2 or MDMX in a native polyacrylamide gel, when inhibitors are added to the complex mixture. Therefore, we propose this new approach to easily screen library of compounds, with potential pharmacological anticancer activity.
Agid:
4789836