Main content area

Phytochemical investigation of Gynura bicolor leaves and cytotoxicity evaluation of the chemical constituents against HCT 116 cells

Teoh, Wuen Yew, Tan, Hooi Poay, Ling, Sui Kiong, Abdul Wahab, Norhanom, Sim, Kae Shin
Natural product research 2016 v.30 no.4 pp. 448-451
4-hydroxybenzoic acid, Gynura bicolor, apoptosis, chemical composition, chlorogenic acid, chromatography, colon, colorectal neoplasms, cytotoxicity, ethyl acetate, guanosine, humans, leaves, neoplasm cells, rutin, vegetables, Malaysia
Gynura bicolor (Compositae) is a popular vegetable in Asia and believed to confer a wide range of benefits including anti-cancer. Our previous findings showed that the ethyl acetate extract of G. bicolor possessed cytotoxicity and induced apoptotic and necrotic cell death in human colon carcinoma cells (HCT 116). A combination of column chromatography had been used to purify chemical constituents from the ethyl acetate and water extract of G. bicolor leaves. Eight chemical constituents 5- p - trans -coumaroylquinic acid (I), 4-hydroxybenzoic acid (II), rutin (III), kampferol-3- O -rutinoside (IV), 3,5-dicaffeoylquinic acid (V), kampferol-3- O -glucoside (VI), guanosine (VII) and chlorogenic acid (VIII) were isolated from G. bicolor grown in Malaysia. To our best knowledge, all chemical constituents were isolated for the first time from G. bicolor leaves except rutin (III). 3,5-dicaffeoylquinic acid (V), guanosine (VII) and chlorogenic acid (VIII) demonstrated selective cytotoxicity (selective index>3) against HCT 116 cancer cells compared to CCD-18Co human normal colon cells.