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Inhibitory Effects of Lanthanum Chloride on Wear Particle-Induced Osteolysis in a Mouse Calvarial Model

Shang, Jiang-Yin-Zi, Zhan, Ping, Jiang, Chuan, Zou, Yang, Liu, Hucheng, Zhang, Bin, Dai, Min
Biological trace element research 2016 v.169 no.2 pp. 303-309
acid phosphatase, bone density, enzyme-linked immunosorbent assay, interleukin-1beta, lanthanum, mice, micro-computed tomography, mineral content, models, resorption, secretion, tumor necrosis factor-alpha
Osteolysis is a bone disorder associated with progressive destruction of bone tissues. However, the effects of lanthanum chloride (LaCl₃) on osteolysis remain unknown. Therefore, the aim of this study was to determine the effects of LaCl₃ on osteolysis in vivo. In a mouse calvarial model, C57BL/6J mice were injected with wear particles with or without LaCl₃. Microcomputed tomography, hematoxylin and eosin staining, and tartrate-resistant acid phosphatase staining were performed for the pathological characterization of calvariae, and eight calvariae per group were prepared for the assay of TNF-α, IL-1β, and RANKL secretion using quantitative enzyme-linked immunosorbent assay (ELISA). In mice treated with high-dose LaCl₃, particle-induced osteolysis and inflammatory reaction were reduced compared with that in the vehicle-treated control. Moreover, treatment with high-dose LaCl₃ suppressed the wear particle-induced decrease in bone mineral content, bone mineral density, and bone volume fraction. Bone destruction and resorption were higher in the LaCl₃-treated group than in the saline-treated group but lower than those in the wear particle group. Finally, our results showed that treatment with a high dose of LaCl₃ suppressed osteoclastogenesis. Thus, LaCl₃ may represent a novel therapeutic agent for the treatment or prevention of wear particle-induced osteolysis and aseptic loosening.