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In Vivo and in Vitro Addition of Dried Olive Extract in Poultry

Author:
King, Annie J., Griffin, Johanna K., Roslan, Fahkirah
Source:
Journal of agricultural and food chemistry 2014 v.62 no.31 pp. 7915-7919
ISSN:
0021-8561
Subject:
GRAS substances, Morella cerifera, Olea europaea, absorbance, adverse effects, antioxidant activity, bark, birds, body weight, diet, feed conversion, feed intake, free radical scavengers, freeze drying, juices, lipid peroxidation, myricetin, olives, organic foods, oxidation, polyphenols, poultry, processed meat, sodium chloride, thiobarbituric acid-reactive substances, trees, unsaturated fatty acids
Abstract:
A freeze-dried powder from organic olive (Olea europaea) juice extract, contains 8.82% polyphenols and a minimum of 2.5% hydroxytyrosol (3,4-dihydroxyphenylethanol), an effective free radical scavenger and the major antioxidant in the byproduct (dried olive extract, DOE). Myricetin, a bioflavonoid extract from the bark powder of the bayberry tree (Myrica cerifera), also has many beneficial biological properties and antioxidative capacity. While well-known as antioxidants, the capacity of these compounds to retard lipid oxidation in foods containing unsaturated fatty acids has not been widely evaluated. Thus, a study was conducted to assess the capacity of DOE to (1) enhance the growth of poultry, (2) determine the effectiveness of DOE (administered in vivo) as an antioxidant in post-mortem tissue and further processed meat, and (3) compare the in vitro antioxidative capacity of hydroxytyrosol and myricetin. DOE was administered ad libitum in water at 6 and 12 mg per bird per day for 6 weeks in a factorial design: 3 diets (control plus two treatment levels) × 2 blocks × 2 replications. There was no enhancement of feed consumption, body weight (BW), or feed conversion by DOE; overall means for these measurements were 5.49 kg per bird, 3.32 kg per bird, and 1.65 g feed per g live BW, respectively. Diagnostic examinations of two birds per pen at the end of the study revealed no adverse effects due to consumption of DOE, a generally recognized as safe substance. The byproduct, administered in vivo, did not retard lipid oxidation in fresh, heated, or NaCl (1.0% w/w)/heated/stored meat as assessed by absorbance values for thiobarbituric acid reactive substances at 532 nm and 2,2-diphenylpicrylhydrazyl at 517 nm. Both the byproduct and hydroxytyrosol are highly water-soluble and may have been unavailable as an antioxidant in the tissue of broilers that did not consume water for 4–6 h prior to processing. As an additive in processed thigh meat, 6 and 12 mg of DOE (2.5% hydroxytyrosol) per 3 mg of meat, although not as effective as myricetin (95% purity), reduced oxidation. Further assessment revealed that hydroxytyrosol from the DOE, added at 1/38 the concentration of myricetin, was almost 50% as effective.
Agid:
4792039