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Production of secondary metabolites by strains of Pseudomonas spp. antagonistic to Colletotrichum falcatum causing red rot disease in sugarcane

Viswanathan, R., Samiyappan, R.
Acta phytopathologica et entomologica Hungarica 2004 v.39 no.1-3 pp. 29-38
Glomerella tucumanensis, Pseudomonas, antibiotics, antifungal properties, conidia, culture media, fungi, germination, indole acetic acid, mycelium, pathogens, pyocyanin, salicylic acid, secondary metabolites, siderophores, sugarcane, systemic acquired resistance, thin layer chromatography
Production of secondary metabolites such as siderophores, indole acetic acid, salicylic acid and antibiotics phenazine, pyocyanine and 2,4-diacetyl phloroglucinol by Pseudomonas spp. associated with induced systemic resistance (ISR) activity in sugarcane against red rot disease was assessed. Many of the strains have been found to produce these metabolites and antibiotics in the culture medium. The antibiotics were separated and identified by thin layer chromatography by running the purified metabolites from the reference strains. The bacterial strains varied in their capability to produce the metabolites. The purified compounds were tested for their antifungal activity against the red rot pathogen Colletotrichum falcatum. Most of the purified metabolites have completely arrested conidial germination and mycelial growth of the fungus. The results suggest that the metabolites production may play a role in antagonism/induced systemic resistance against the pathogen.