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Identification of microsatellite markers linked to powdery mildew resistance gene <i>Pm2</i> in wheat
- Qiu, Y. C., Sun, X. L., Zhou, R. H., Kong, X. Y., Zhang, S. S., Jia, J. Z.
- Cereal research communications 2006 v.34 no.4 pp. 1267-1273
- disease resistance, genes, genetic distance, genetic markers, loci, marker-assisted selection, microsatellite repeats, powdery mildew, wheat, China
- The powdery mildew resistance gene <i>Pm2</i> is effective in China. Bulked segregant analysis (BSA) was used to search for microsatellite markers linked to <i>Pm2</i> . Twenty-one microsatellite primer pairs located on chromosome 5DS were screened; three polymorphic loci <i>Xcfd81</i> -5DS, <i>Xgwm190</i> -5DS, and <i>Xcfd18</i> -5DS were linked to <i>Pm2</i> using an F <sub>2</sub> population from Chinese Spring × C114118 (with <i>Pm2</i>) consisting of 814 individuals. The genetic distances between <i>Pm2</i> and the three markers were: 2.0cM, 34.2cM and 44.2cM, respectively. Microsatellite marker <i>Xcfd81</i> -5DS could be used in marker assisted selection for <i>Pm2</i> provided any chosen <i>Pm2</i> source also carries the relevant marker.