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Isolation and analysis of tetracycline-resistant <i>Mycoplasma agalactiae</i> strains from an infected goat herd in Cyprus — short communication

Filioussis, George, Ioannou, Ioannis, Petridou, Evanthia, Avraam, Maria, Giadinis, Nektarios D., Kritas, Spyridon K.
Acta veterinaria Hungarica 2013 v.61 no.3 pp. 291-296
Mycoplasma agalactiae, chromosome aberrations, enrofloxacin, genes, goats, herds, lincomycin, microorganisms, phenotype, polymerase chain reaction, spiramycin, tetracycline, tylosin, Cyprus
A major concern with the use of tetracycline against mycoplasmas is the development of resistance. Infections in small ruminants due to tetracyclineresistant <i>Mycoplasma agalactiae</i> strains are becoming a frequent problem worldwide. In the present paper the detection and analysis of three tetracycline-resistant <i>M. agalactiae</i> strains, isolated from infected goats in Cyprus, are reported. The three field isolates were identified as <i>M. agalactiae</i> by polymerase chain reaction (PCR) showing 98% identity to the <i>M. agalactiae</i> PG2 reference strain. Furthermore, they were found sensitive to tylosin, enrofloxacin, spiramycin and lincomycin. In contrast, they were resistant to tetracycline. None of the putative genes [<i>tet</i>(M), <i>tet</i>(O) and <i>tet</i>(S)] that commonly contribute to high-level resistance to tetracycline could be amplified from their genome. Contrarily, the field isolates were found to carry IS<i>Mag1</i>, an insertion sequence related to the IS30 family of mobile elements. Although IS<i>Mag1</i> is widely believed to induce high-frequency chromosomal rearrangements resulting in phenotypic changes of microorganisms, its potential role in tetracycline resistance of mycoplasmas requires further studies.