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Example of the application the microsatellite DNA fragments in the study of farmed European catfish (Silurus glanis, L.) broodstock

Kuciñski, Marcin, Liszewski, Tomasz, Krzyoeków, Anna, Fopp-Bayat, Dorota, Łączyñska, Beata
Archiwum rybactwa polskiego = 2015 v.23 no.2 pp. 91-99
DNA fragmentation, Silurus glanis, breeding stock, farmers, freshwater aquaculture, freshwater fish, genetic techniques and protocols, genetic variation, inbreeding coefficient, microsatellite repeats, population size, spawning, spermatozoa, Poland
European catfish, Silurus glanis L., is the second largest freshwater fish in Europe. The species is very popular among the farmers, as it is one of the most promising European aquaculture species. Despite the growing importance of European catfish in freshwater aquaculture, the genetic data available on this species are still limited. The main purpose of the present study was to develop a reliable, feasible genetic protocol for future studies on European catfish populations and broodstocks in Poland. The genetic characteristics of the tested fish group were based on genetic parameters such as the polymorphism information content (PIC), the effective population size (Ne), the inbreeding coefficient (Fis), and the Garza-Williamson index (M), among others. Additionally, the potential effects of a genetic bottleneck on the genetic variation of the broodstock were examined. The genetic analysis protocol described in this study can be used to establish genetic-based records for European catfish broodstocks, including for sperm cryobanking. This approach will be useful for elaborating the selection procedures that allow for optimal assemblages of spawning pairs in artificial reproduction. The application of the genetic analysis protocol in practice will permit maintaining high quality in European catfish broodstocks.