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Spasmolytic activity of Rosmarinus officinalis L. involves calcium channels in the guinea pig ileum

Ventura-Martínez, Rosa, Rivero-Osorno, Oscar, Gómez, Claudia, González-Trujano, María Eva
Journal of ethnopharmacology 2011 v.137 no.3 pp. 1528-1532
Rosmarinus officinalis, acetylcholine, aerial parts, calcium channel blockers, calcium channels, electric field, ethanol, guinea pigs, ileum, mechanism of action, nitric oxide, nitric oxide synthase, pain, papaverine, potassium chloride, prostaglandins, receptors, smooth muscle, traditional medicine
ETHNOPHARMACOLOGICAL RELEVANCE: Rosmarinus officinalis L. is a plant used around the world for its properties to cure pain in several conditions, such as arthritic and abdominal pain or as an antispasmodic; however, there are no scientific studies demonstrating its spasmolytic activity. Therefore, the aim of the present study was to investigate the effect of an ethanol extract from Rosmarinus officinalis aerial parts and the possible mechanism involved by using rings from the isolated guinea pig ileum (IGPI). MATERIALS AND METHODS: The IGPI rings were pre-contracted with potassium chloride (KCl; 60mM), acetylcholine (ACh; 1×10⁻⁹ to 1×10⁻⁵M) or electrical field stimulation (EFS; 0.3Hz of frequency, 3.0ms of duration and 14V intensity) and tested in the presence of the Rosmarinus officinalis ethanol extract (150, 300, 600 and 1 200μg/mL) or a referenced smooth muscle relaxant (papaverine, 30μM). In addition, the possible mechanism of action was analyzed in the presence of hexametonium (a ganglionic blocker), indomethacine (an inhibitor of prostaglandins), l-NAME (a selective inhibitor of the nitric oxide synthase) and nifedipine (a calcium channel blocker). RESULTS: Rosmarinus officinalis ethanol extract exhibited a significant and concentration-dependent spasmolytic activity on the contractions induced by KCl (CI₅₀=661.06±155.91μg/mL); ACh (CI₅₀=464.05±16.85μg/mL) and EFS (CI₅₀=513.72±34.13μg/mL). Spasmolytic response of Rosmarinus officinalis (600μg/mL) was reverted in the presence of nifedipine 1μM, but not in the presence of hexamethonium 0.5mM, indomethacine 1μM or l-NAME 100μM. CONCLUSION: The present results reinforce the use of Rosmarinus officinalis as antispasmodic in folk medicine. Moreover, it is demonstrated the involvement of calcium channels in this activity, but not the participation of nicotinic receptors, prostaglandins or nitric oxide.