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Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Nadăş, George Cosmin, Kalmár, Zsuzsa, Taulescu, Marian Aurel, Chirilă, Flore, Bouari, Cosmina Maria, Răpuntean, Sorin, Bolfa, Pompei, Fiţ, Nicodim Iosif
Bulletin of the Veterinary Institute in Puławy = 2014 v.58 no.2 pp. 219-222
Candida albicans, Candida kefyr, Candida krusei, Candida parapsilosis, Candida saitoana, animals, genotyping, polymerase chain reaction, restriction fragment length polymorphism, species identification, strains
The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.