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Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Author:
Nadăş, George Cosmin, Kalmár, Zsuzsa, Taulescu, Marian Aurel, Chirilă, Flore, Bouari, Cosmina Maria, Răpuntean, Sorin, Bolfa, Pompei, Fiţ, Nicodim Iosif
Source:
Bulletin of the Veterinary Institute in Puławy = 2014 v.58 no.2 pp. 219-222
Subject:
Candida albicans, Candida kefyr, Candida krusei, Candida parapsilosis, Candida saitoana, animals, genotyping, polymerase chain reaction, restriction fragment length polymorphism, species identification, strains
Abstract:
The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.