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FERMENTATION OF A NEW Bacillus thuringiensis STRAIN / FERMENTACION DE LA VARIEDAD NUEVA Bacillus thuringiensis / FERMENTATION DE LA NOUVELLE RACE Bacillus thuringiensis
- Bai, Cheng, Vick, Brady A.
- Helia 2013 v.24 no.35 pp. 47-54
- Bacillus thuringiensis, Cochylis hospes, Helianthus annuus, bioreactors, fermentation, harvesting, instars, lethal concentration 50, pests, North America
- A new Bacillus thuringiensis strain, 00-50-5, exhibited an unusually high activity against banded sunflower moth (BSM) Cochylis hospes, which is an economically important pest of cultivated sunflowers in North America. The strain was fermented with a modified commercial medium in a bioreactor. This was a successful trial for production of 00-50-5 on a larger scale. After fermentation, the yield of dried powder was 5.8 g/l broth. In the laboratory assays, the LC₅₀ (lethal concentration required to kill 50% of targets) of the dried powder was 0.5 μg/ml 3 d after exposure against four instars of BSM. Some practically useful conditions and parameters for the fermentation operating procedure and methods for harvesting were determined.