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Effects of abscisic acid and an osmoticum on the maturation, starch accumulation and germination of Picea spp. somatic embryos

Hazubska-Przybył, Teresa, Kalemba, Ewa M., Ratajczak, Ewelina, Bojarczuk, Krystyna
Acta physiologiae plantarum 2016 v.38 no.2 pp. 59
Picea abies, Picea omorika, abscisic acid, germination, hypocotyls, somatic embryos, starch, sucrose, tissues
The aim of this study was to investigate whether ABA, sucrose or Phytagel applied to the maturation medium at different concentrations affected the growth, development and starch content of somatic embryos of Picea abies and Picea omorika. Embryogenic tissues of both spruce species were placed on maturation medium supplemented with various doses of ABA (10, 20, 40 and 80 µM), sucrose (17, 34 and 68 g/L) or Phytagel (4, 6 and 8 g/L). Our results showed that ABA and the osmoticum had a significant effect on the production and maturation of somatic embryos of both spruce species. Supplementing the medium with low concentrations of ABA (10 µM) or sucrose (17 g/L) resulted in the precocious germination of embryos. Adding sucrose to the maturation medium at the highest tested concentration (68 g/L) improved the growth of the radicles of the embryos of both spruce species during the germination stage. Moreover, the intensity of the growth of the hypocotyls and radicles of P. omorika germinating embryos depended on the Phytagel concentration applied to the maturation medium. The starch content and the starch accumulation pattern of Picea abies and P. omorika somatic embryos were dependent on the concentration of ABA, sucrose or Phytagel in the maturation medium. In general, during development, the P. abies somatic embryos accumulated a much greater amount of starch than P. omorika embryos did. The effects of ABA and osmoticum on the starch accumulation pattern of Picea abies and P. omorika somatic embryos are first reported in this paper.