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Effects of temperature and pretreatment conditions on mixed-acid fermentation of water hyacinths using a mixed culture of thermophilic microorganisms
- Forrest, Andrea K., Hernandez, Joan, Holtzapple, Mark T.
- Bioresource technology 2010 v.101 no.19 pp. 7510-7515
- Eichhornia crassipes, acids, biomass, economic valuation, feedstocks, fermentation, freshwater, fuels, limes, mixed culture, salts, temperature, thermophilic microorganisms, waterways
- The MixAlco™ process biologically converts biomass to carboxylate salts that may be chemically converted to a wide variety of chemicals and fuels. This study investigated the use of water hyacinths as a feedstock, comparing digestibility after each of four different pretreatments at two fermentation temperatures (40 and 55°C). Water hyacinths were treated with excess lime (0.3g Ca(OH)₂/g dry biomass). Short-term treatment occurred for 1 and 2h at 100°C. Long-term treatment occurred for 4 and 6weeks at 50°C. Treated water hyacinths were fermented with marine microorganisms for 28days and acid concentration (g/L), conversion (g volatile solids (VS) digested/g VS fed), and selectivity (g acid/g VS digested) were measured. All pretreatments out performed fresh feedstock fermentations. The 40°C fermentations exhibited greater acid yields and selectivity than the 55°C. The 1-h hot-lime pretreatment exhibited the best overall outcomes at approximately 250%, 200%, and 125% increases relative to the fresh water hyacinths in total acid, conversion, and selectivity, respectively. The results show that with a gentle 1-h hot-lime pretreatment, water hyacinths can be fermented to produce liquid fuels, thus creating an economic value to water hyacinths that are cleared from choked waterways.