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Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Author:
Botcherby, Edward J., Smith, Christopher W., Kohl, Michael M., Débarre, Delphine, Booth, Martin J., Juškaitis, Rimas, Paulsen, Ole, Wilson, Tony
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 2919-2924
ISSN:
0027-8424
Subject:
image analysis, microscopy, neurons
Abstract:
Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
Agid:
484364