Jump to Main Content
Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates
- Botcherby, Edward J., Smith, Christopher W., Kohl, Michael M., Débarre, Delphine, Booth, Martin J., Juškaitis, Rimas, Paulsen, Ole, Wilson, Tony
- Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 2919-2924
- image analysis, microscopy, neurons
- Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.