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Saccharomyces cerevisiae decreases inflammatory responses induced by F4⁺ enterotoxigenic Escherichia coli in porcine intestinal epithelial cells

Zanello, Galliano, Meurens, François, Berri, Mustapha, Chevaleyre, Claire, Melo, Sandrine, Auclair, Eric, Salmon, Henri
Veterinary immunology and immunopathology 2011 v.141 no.1-2 pp. 133-138
Saccharomyces cerevisiae, cell culture, chemokines, diarrhea, enterotoxigenic Escherichia coli, epithelial cells, gene expression, inflammation, messenger RNA, pathogens, piglets, probiotics, yeasts
Probiotic yeasts may provide protection against intestinal inflammation induced by enteric pathogens. In piglets, infection with F4⁺ enterotoxigenic Escherichia coli (ETEC) leads to inflammation, diarrhea and intestinal damage. In this study, we investigated whether the yeast strains Saccharomyces cerevisiae (Sc, strain CNCM I-3856) and S. cerevisiae variety boulardii (Sb, strain CNCM I-3799) decreased the expression of pro-inflammatory cytokines and chemokines in intestinal epithelial IPI-2I cells cultured with F4⁺ ETEC. Results showed that viable Sc inhibited the ETEC-induced TNF-α gene expression whereas Sb did not. In contrast, killed Sc failed to inhibit the expression of pro-inflammatory genes. This inhibition was dependent on secreted soluble factors. Sc culture supernatant decreased the TNF-α, IL-1α, IL-6, IL-8, CXCL2 and CCL20 ETEC-induced mRNA. Furthermore, Sc culture supernatant filtrated fraction <10kDa displayed the same effects excepted for TNF-α. Thus, our results extended to Sc (strain CNCM I-3856) the inhibitory effects of some probiotic yeast strains onto inflammation.