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Saccharomyces cerevisiae decreases inflammatory responses induced by F4⁺ enterotoxigenic Escherichia coli in porcine intestinal epithelial cells

Author:
Zanello, Galliano, Meurens, François, Berri, Mustapha, Chevaleyre, Claire, Melo, Sandrine, Auclair, Eric, Salmon, Henri
Source:
Veterinary immunology and immunopathology 2011 v.141 no.1-2 pp. 133-138
ISSN:
0165-2427
Subject:
Saccharomyces cerevisiae, cell culture, chemokines, diarrhea, enterotoxigenic Escherichia coli, epithelial cells, gene expression, inflammation, messenger RNA, pathogens, piglets, probiotics, yeasts
Abstract:
Probiotic yeasts may provide protection against intestinal inflammation induced by enteric pathogens. In piglets, infection with F4⁺ enterotoxigenic Escherichia coli (ETEC) leads to inflammation, diarrhea and intestinal damage. In this study, we investigated whether the yeast strains Saccharomyces cerevisiae (Sc, strain CNCM I-3856) and S. cerevisiae variety boulardii (Sb, strain CNCM I-3799) decreased the expression of pro-inflammatory cytokines and chemokines in intestinal epithelial IPI-2I cells cultured with F4⁺ ETEC. Results showed that viable Sc inhibited the ETEC-induced TNF-α gene expression whereas Sb did not. In contrast, killed Sc failed to inhibit the expression of pro-inflammatory genes. This inhibition was dependent on secreted soluble factors. Sc culture supernatant decreased the TNF-α, IL-1α, IL-6, IL-8, CXCL2 and CCL20 ETEC-induced mRNA. Furthermore, Sc culture supernatant filtrated fraction <10kDa displayed the same effects excepted for TNF-α. Thus, our results extended to Sc (strain CNCM I-3856) the inhibitory effects of some probiotic yeast strains onto inflammation.
Agid:
485723