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Discrimination of rapeseeds (Brassica napus L.) based on the content of erucic acid by 1H NMR

Han, Jiao, Lu, Changming, Li, Yinping, Deng, Zhiwei, Fu, Boqiang, Geng, Zhufeng
European food research & technology 2016 v.242 no.3 pp. 441-447
Brassica napus, cluster analysis, erucic acid, food research, gas chromatography, human health, nuclear magnetic resonance spectroscopy, rapeseed, rapid methods, screening, t-test
Rapeseeds (Brassica napus L.) are major source of edible oil-consumed in daily life. However, the high erucic acid content in some rapeseeds is harmful to the human health. So it is essential to explore a convenient and rapid method to identify which content of erucic acid is less than 5 %. Since the derivation procedure of traditional identification method (gas chromatography) is cumbersome, it consumes much time in analysis. In this work, a rapid method based on nuclear magnetic resonance (NMR) technology is developed. Firstly, ¹H NMR spectra of rapeseeds extract were collected, and then ¹H NMR data were analyzed by hierarchical cluster analysis and t test. The results indicated that statistically significant differences (p < 0.05) were found between high-erucic-acid and low-erucic-acid groups. Therefore, the new method can effectively distinguish LEAR from high-erucic-acid rapeseed with convenient sample pretreatment and data acquisition, which offer great potential for LEAR screening.