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Ciprofloxacin-resistant Escherichia coli in hospital wastewater of Bangladesh and prediction of its mechanism of resistance

Author:
Akter, Farhima, Amin, M. Ruhul, Osman, Khan Tanjid, Anwar, M. Nural, Karim, M. Manjurul, Hossain, M. Anwar
Source:
World journal of microbiology & biotechnology 2012 v.28 no.3 pp. 827-834
ISSN:
0959-3993
Subject:
DNA topoisomerase (ATP-hydrolysing), Escherichia coli, antibiotic resistance, antibiotics, bacteria, ciprofloxacin, genes, mutation, plasmids, pollution, prediction, wastewater, Bangladesh
Abstract:
Hospital and agriculture wastewater is mostly responsible for causing environmental pollution by spreading un-metabolized antibiotics and resistant bacteria, especially in Bangladesh. Here, we studied the influence of the most frequently prescribed antibiotic, fluoroquinolone (~72%), on the development of antibiotic resistance in Escherichia coli. Out of 300, 24 ciprofloxacin resistant E. coli isolates were selected for the study that showed the MBC100 higher than expected (600 μg/mL). Here, we profiled plasmid, sequenced gyr genes, screened mutations and analyzed the effect of mutation on drug-protein interaction through molecular docking approach. We found that (1) out of 10, most of them (n = 7) had large plasmid(s); (2) all ciprofloxacin-resistant isolates had gyrA double mutations (S83L and D87Y); (3) no isolate had qnr gene; and (4) docking of ciprofloxacin with DNA gyrase A subunit suggests that acquisition of double mutation leads to alteration of the ciprofloxacin binding pocket.
Agid:
487423