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Partial retrotransposon-like DNA sequence in the genomic clone of Aspergillus flavus, pAF28
- Okubara, Patricia A., Tibbot, Brian K., Tarun, Alice S., McAlpin, Cesaria E., Hua, Sui-Sheng T.
- Mycological research 2003 v.107 pp. 841–846
- Aspergillus flavus, retrotransposons, transposons, sequence analysis, genetic polymorphism, host-pathogen relationships, Zea mays, nut trees
- A genomic clone of the aflatoxin-producing fungus Aspergillus flavus, designated pAF28, has been used as a probe for Southern blot fingerprinting of fungal strains. A large number of A. flavus strains isolated from corn fields and tree-nut orchards can be distinguished because the DNA fingerprint patterns are highly polymorphic. We have completed the sequencing of a 6355 bp insert in pAF28. The sequence features motifs and open reading frames characteristic of transposable elements of the gypsy class. We have named this new element AfRTL-1, for A. flavus retrotransposon-like DNA.