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Microsatellite markers for the giant kelp Macrocystis pyrifera

Author:
Alberto, Filipe, Whitmer, Allison, Coelho, Nélson C., Zippay, Mackenzie, Varela-Alvarez, Elena, Raimondi, Peter T., Reed, Daniel C., Serrão, Ester A.
Source:
Conservation genetics 2009 v.10 no.6 pp. 1915-1917
ISSN:
1566-0621
Subject:
Macrocystis pyrifera, alleles, ecology, genomic libraries, loci, macroalgae, microsatellite repeats, population genetics, screening
Abstract:
We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.
Agid:
490336