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Salinicoccus kekensis sp. nov., a novel alkaliphile and moderate halophile isolated from Keke Salt Lake in Qinghai, China

Gao, Miao, Wang, Lei, Chen, San-feng, Zhou, Yu-guang, Liu, Hong-can
Antonie van Leeuwenhoek 2010 v.98 no.3 pp. 351-357
DNA, Salinicoccus, fatty acids, lakes, nucleic acid hybridization, nucleotide sequences, pH, phylogeny, ribosomal RNA, sodium chloride, China
A novel alkaliphilic and moderate halophilic bacterium, designated strain K164T, was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 2.0-20.0% (w/v) NaCl, at 4-50°C and pH 6.5-11.5, with an optimum of 8% (w/v) NaCl, 37°C and pH 10, respectively. The predominant respiratory quinone was menaquinone 6 (MK-6) and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were anteiso-C₁₅:₀ and iso-C₁₅:₀. The genomic DNA G+C content was 50.16 mol. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K164T was a member of the genus Salinicoccus. Strain K164T showed the highest similarity (98.4%) with Salinicoccus alkaliphilus AS 1.2691T and below 97% similarity with other recognized members of the genus in 16S rRNA gene sequence. Level of DNA-DNA relatedness between strain K164T and Salinicoccus alkaliphilus AS 1.2691T was 20.1%. On the basis of its phenotypic characteristics and the level of DNA-DNA hybridization, strain K164T is considered to represent a novel species of the genus Salinicoccus, for which the name Salinicoccus kekensis sp. nov. is proposed. The type strain is K164T (=CGMCC 1.10337T = DSM 23173T).