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Fractional pretreatment of hybrid poplar for accelerated enzymatic hydrolysis: Characterization of cellulose-enriched fraction

Meng, Ling Yan, Kang, Su Min, Zhang, Xue Ming, Wu, Yu Ying, Xu, Feng, Sun, Run Cang
Bioresource technology 2012 v.110 pp. 308-313
biomass, biopolymers, cellulases, cellulose, dimethyl sulfoxide, enzymatic hydrolysis, ethanol production, glucose, hybrids, lithium chloride, new methods, nuclear magnetic resonance spectroscopy, scanning electron microscopy, spectral analysis, wood, xylose
The efficient production of fermentable sugars is a prerequisite for economic ethanol production from lignocellulosic biomass. A cellulose-enriched fraction was obtained by complete dissolution of ball-milled hybrid poplar wood in dimethyl sulfoxide and lithium chloride (DMSO/LiCl). The cellulose-enriched fraction was mainly composed of glucose and xylose. Spectral fitting analysis of CP/MAS ¹³C NMR revealed that the cellulose-enriched fraction had para-crystalline structures with somewhat larger contents of crystalline cellulose Іᵦ than Іα. The cellulose-enriched fraction was much easier to be converted into mono sugars by cellulases than the untreated sample. Scanning electron microscopy (SEM) indicated that the cellulose-enriched fraction was porous, which could likely explain the high enzymatic hydrolysis efficiency. This study provides a novel pretreatment method based on fractional separation of the three biopolymers via complete dissolution system for enhancement of conversion from ignocellulose to biofuels.