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Proof of Concept for Recombinant Cellular Controls in Quantitative Molecular Pathogen Detection
- Rossmanith, Peter, Mester, Patrick, Frühwirth, Karin, Fuchs, Sabine, Wagner, Martin
- Applied and environmental microbiology 2011 v.77 no.7 pp. 2531-2533
- Listeria monocytogenes, pathogens, polymerase chain reaction, process control
- In this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinant Listeria monocytogenes ΔprfA (targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC.