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Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

Author:
Jäpelt, Rie Bak, Silvestro, Daniele, Smedsgaard, Jørn, Jensen, Poul Erik, Jakobsen, Jette
Source:
Food chemistry 2013 v.138 no.2-3 pp. 1206-1211
ISSN:
0308-8146
Subject:
Capsicum annuum, Solanum glaucophyllum, Solanum lycopersicum, derivatization, heat treatment, ionization, leaves, liquid chromatography, mass spectrometry, metabolites, sweet peppers, tomatoes, vitamins
Abstract:
Changes in vitamin D3 and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf., Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC–ESI-MS/MS) method including Diels–Alder derivatisation. Vitamin D3 and 25-hydroxy vitamin D3 were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200ng vitamin D3/g dry weight and 31ng 25-hydroxy vitamin D3/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D3 in both free (32ng/g dry weight) and glycosylated form (17ng/g dry weight).
Agid:
493873