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Simultaneous quantification of flavonoids and phenolic acids in Herba Scutellariae barbatae and its confused plants by high performance liquid chromatography-tandem mass spectrometry

Author:
Qiao, Shi, Shi, Rui, Liu, Man, Zhang, Chong, Yang, Wei, Shi, Xiaowei, Jiang, Xijuan, Wang, Chunying, Wang, Qiao
Source:
Food chemistry 2011 v.129 no.3 pp. 1297-1304
ISSN:
0308-8146
Subject:
acetic acid, detection limit, flavonoids, hybrids, ions, mass spectrometry, methanol, monitoring, phenolic acids, plants, quantitative analysis, spectrometers
Abstract:
A sensitive and selective high performance liquid chromatography–tandem mass spectrometric method (HPLC–MS/MS) has been developed for simultaneous analysis of 11 components (7 flavonoids and 4 phenolic acids) in Herba Scutellariae barbatae. Simultaneous separation of these 11 compounds was achieved on a C₁₈ analytical column with gradient elution of methanol and 0.1‰ acetic acid (v/v) at a flow rate of 0.8mL/min. The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer equipped with a turbo ion spray interface in negative mode using multiple-reaction monitoring (MRM). Full validation of the assay was carried out including linearity, precision, accuracy, limits of detection and quantification. The results demonstrated that the method developed was reliable, rapid and specific. Then the method was successfully applied to differentiate 18 batches of Herba Scutellariae barbatae samples and 3 batches of its confused plants from different sources.
Agid:
495214