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Antiviral activity of bovine type III interferon against foot-and-mouth disease virus

Author:
Díaz-San Segundo, Fayna, Weiss, Marcelo, Perez-Martín, Eva, Koster, Marla J., Zhu, James, Grubman, Marvin J., de los Santos, Teresa
Source:
Virology 2011 v.413 no.2 pp. 283
ISSN:
0042-6822
Subject:
immune response, interferons, gene expression regulation, antiviral properties, Foot-and-mouth disease virus, foot-and-mouth disease, cattle diseases, host-pathogen relationships
Abstract:
Foot-and-mouth disease (FMD) is one of the most serious threats to the livestock industry. Despite the availability of a vaccine, recent outbreaks in disease-free countries have demonstrated that development of novel FMD control strategies is imperative. Here we report the identification and characterization of bovine (bo) interferon lambda 3 (IFN-λ3), a member of the type III IFN family. Expression of boIFN-λ3 using a replication-defective human adenovirus type 5 vector (Ad5-boIFN-λ3) yielded a glycosylated secreted protein with antiviral activity against FMD virus (FMDV) and vesicular stomatitis virus in bovine cell culture. Inoculation of cattle with Ad5-boIFN-λ3 induced systemic antiviral activity and up-regulation of IFN stimulated gene expression in multiple tissues susceptible to FMDV infection. Our results demonstrate that the type III IFN family is conserved in bovines and boIFN-λ3 has potential for further development as a biotherapeutic candidate to inhibit FMDV or other viruses in cattle.
Agid:
49642
Handle:
10113/49642