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A mariner-Based Transposition System for Listeria monocytogenes

Author:
Cao, Min, Bitar, Alan Pavinski, Marquis, Hélène
Source:
Applied and environmental microbiology 2007 v.73 no.8 pp. 2758-2761
ISSN:
0099-2240
Subject:
Listeria monocytogenes, mutagenesis, plasmids, transposition (genetics), transposons
Abstract:
In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes.
Agid:
496743