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De novo sequencing and development of EST-SSR for Gevuina avellana (Proteaceae), a nut tree from South America

Riegel, Ricardo, Diaz, Leila, Véliz, Débora
American journal of botany 2010 v.97 no.12 pp. e133
Baccharis, Proteaceae, alleles, cDNA libraries, clones, complementary DNA, expressed sequence tags, genetic variation, hazelnuts, heterozygosity, loci, microsatellite repeats, nut trees, South America
Premise of the study: The aim of this study was to develop molecular tools to investigate the genetic structure and diversity of natural populations of Gevuina avellana (Molina, Proteaceae), the Chilean hazelnut. Simple sequence repeats (SSRs) derived from expressed sequence tags (EST-SSRs) were developed, optimized, and characterized. METHODS: and Results: The microsatellite-containing sequences were selected from a cDNA library developed from the nut. The eight marker loci showed two to seven alleles in 60 unrelated trees, from two different natural populations. The observed heterozygosity (Ho) was 0.44, ranging from 0.07 to 0.92 for different loci. When the multilocus genotype of the eight EST-SSRs was considered, all trees could be differentiated. CONCLUSIONS: The newly identified EST-SSRs in G. avellana and the multi-pooling strategy involving eight markers will facilitate future studies of clone identification, genetic structure, and diversity.