Main content area

A set of near-isogenic lines for blast resistance genes with an Indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background

Koide, Yohei, Ebron, Leodegario A., Kato, Hiroshi, Tsunematsu, Hiroshi, Telebanco-Yanoria, Mary Jeanie, Kobayashi, Nobuya, Yokoo, Masao, Maruyama, Sachio, Imbe, Tokio, Fukuta, Yoshimichi
Field crops research 2011 v.123 no.1 pp. 19-27
Oryza sativa, backcrossing, blast disease, chromosomes, disease resistance, genes, genetic background, genetic markers, introgression, isogenic lines, lowlands, rice, surveys, tropics, Philippines
A set of near-isogenic lines for blast resistance genes was developed by using an Indica-type elite rice variety, IR49830-7-1-2-2, suitable for the rainfed lowland conditions in the tropics, as a genetic background. Initially, we revealed that IR49830-7-1-2-2 harbors five blast resistance genes –Pia, Pib, Pik-s, Pita, and Pi11(t) – by using a differential system involving 19 selected standard blast isolates from the Philippines. Based on this result, we developed nine near-isogenic lines (NILs) targeting eight resistance genes –Pik, Pi7(t), Pi3, Pi5, Pita-2, Piz-5, Pish, and Pi9 – by recurrent backcrossing. The introgression of each resistance gene in the NILs was confirmed by reaction patterns to the blast isolates, allelism tests, and DNA marker analysis. In addition, a genome-wide DNA marker survey revealed that most of the chromosome regions in each NIL were of the IR49830-7-1-2-2 type. The agricultural characteristics of most of the developed NILs were almost the same as those of IR49830-7-1-2-2. Moreover, with one exception, they showed submergence tolerance similar to IR49830-7-1-2-2. The developed NILs could be used as a multiline variety suitable for the rainfed lowland in the tropics.